Characterization of exo-s, exo-u, and alg virulence factors and antimicrobial resistance in Pseudomonas aeruginosa isolated from migratory Egyptian vultures from India
نویسندگان
چکیده
This study of Pseudomonas aeruginosa in fecal droppings of migratory Egyptian vultures (Neophron p. percnopterus) revealed eight positive samples (n=25) by a 16S rRNA gene-based PCR in two consecutive winter seasons. Disk diffusion sensitivity testing revealed three multiple antimicrobial resistant (MAR) isolates. Genotypic characterization showed mutually exclusive exo-s and exo-u virulence genes in five and three isolates, respectively, while the alg gene was present in all of the isolates. MAR isolates with virulence genes were detected in both seasons. The Egyptian vultures could potentially be vectors of pathogenic and MAR P. aeruginosa, thereby affecting regional control and preventive measures.
منابع مشابه
Genotyping of Pseudomonas aeruginosa strains as a multidrug resistant (MDR) bacterium and evaluating the prevalence of ESBLs and some virulence factors encoding genes by PFGE and ERIC-PCR methods
Pseudomonas aeruginosa is an important multi-drug resistant (MDR) opportunistic bacterium. 102 strains of Pseudomonas aeruginosa equally isolated from human and cow milk were subjected to Multiplex-PCR for detection of ESBLs and exoenzymes of U, T, S, OprI, and OprL, Integrons class A encoding genes and genotyping by the ERIC-PCR and PFGE methods. The disc diffusion and E-test based on CLSI (Cl...
متن کاملGenotyping of Pseudomonas aeruginosa strains as a multidrug resistant (MDR) bacterium and evaluating the prevalence of ESBLs and some virulence factors encoding genes by PFGE and ERIC-PCR methods
Pseudomonas aeruginosa is an important multi-drug resistant (MDR) opportunistic bacterium. 102 strains of Pseudomonas aeruginosa equally isolated from human and cow milk were subjected to Multiplex-PCR for detection of ESBLs and exoenzymes of U, T, S, OprI, and OprL, Integrons class A encoding genes and genotyping by the ERIC-PCR and PFGE methods. The disc diffusion and E-test based on CLSI (Cl...
متن کاملGenetic features of clinical Pseudomonas aeruginosa strains.
The genetic features of each isolate were determined by enterobacterial repetitive intergenic consensus (ERIC) primer sequences used in PCR and by searching for six virulence genes (alg D, las B, tox A, plc H, plc N, exo S). 49 (79%) of the isolates were distributed in three ERIC PCR subgroups and showed 62% of similarity. The remaining 13 strains generated unique patterns. The first subgroup w...
متن کاملReal- Time - RT PCR Study of Aqueous Extract of Ammi visnaga on Pseudomonas aeruginosa Exo A and Exo S Genes Expression
Background and Aim: P. aeruginosa exotoxins are responsible for pathogenicity and antibiotic resistant. The aim of this study was to investigate the effects of the Ammi. visnaga (A. visnaga) extract on the P. aeruginosa exotoxin A and S genes expression. Materials and Methods: Antibacterial effects of the A. visnaga ethanolic extract were performed using the micro dilution technique in sequent...
متن کاملAntibiotic resistance profiles of Pseudomonas aeruginosa isolates containing virulence genes
Background: A most common opportunistic pathogen, Pseudomonas aeruginosa is present in both humans and animals and responsible for various nosocomial infections and healthcare settings related infections. Different virulence genes like; oprL (membrane lipoprotein L) and toxA (exotoxin A i.e. ETA) in P. aeruginosa, assist in its pathogenicity, toxicity and contribute to high antibiotic resistanc...
متن کامل